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Garnier, Matthieu; Labreuche, Yannick; Nicolas, Jean-louis. |
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within... |
Tipo: Text |
Palavras-chave: Polyphasic approach; Summer mortalities; Crassostrea gigas; Pathogen; French isolates; Vibrio aestuarianus. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4961.pdf |
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